Literature

Publications of
ISPMF Members

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2021

Alvisi, N., van Noort, K., Dwiani, S., Geschiere, N., Sukarta, O., Varossieau, K., Nguyen, DL., Strasser, R., Hokke, CH., Schots, A., Wilbers, RHP. (2021) β-Hexosaminidases Along the Secretory Pathway of Nicotiana benthamiana Have Distinct Specificities Toward Engineered Helminth N-Glycans on Recombinant Glycoproteins. Frontiers in Plant Science 12, 638454
Buyel, JF., Stöger, E., Bortesi, L. (2021) Targeted genome editing of plants and plant cells for biomanufacturing. Transgenic Research, first published on 1/03/2021 (doi: 10.1007/s11248-021-00236-z)
Castells-Graells, R., Lomonossoff, GP. (2021) Plant-based production can result in covalent cross-linking of proteins. Plant Biotechnology Journal, first published on 8/04/2021 (doi: 10.1111/pbi.13598)
Castells-Graells, R., Ribeiro, JRS., Domitrovic, T., Hesketh, EL., Scarff, C.A., Johnson, JE., Ranson, N.A., Lawson, DM., Lomonossoff, GP. (2021) Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus. Communications Biology 4, 619
Chin-Fatt, A., Menassa, R. (2021) A VHH-Fc Fusion Targeted to the Chloroplast Thylakoid Lumen Assembles and Neutralizes Enterohemorrhagic E. coli O157:H7. Frontiers in Plant Science 12, 686421
Chin-Fatt, A., Saberianfar, R., Menassa, R. (2021) A Rationally Designed Bovine IgA Fc Scaffold Enhances in planta Accumulation of a VHH-Fc Fusion Without Compromising Binding to Enterohemorrhagic E. coli. Frontiers in Plant Science 12, 651262
Frías-Sánchez AI, Quevedo-Moreno DA, Samandari M, Tavares Negrete JA, Sánchez-Rodríguez VH, González-Gamboa I, Ponz F, Alvarez MM, Trujillo de Santiago G. (2021) Biofabrication of muscle fibers enhanced with plant viral nanoparticles using surface chaotic flows. Biofabrication, first published on 8/01/2021 (doi: 10.1088/1758-5090/abd9d7)
Herman, X., Far, J., Courtoy, A., Bouhon, L., Quinton, L., De Pauw, E., Chaumont, F., Navarre, C. (2021) Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans. Frontiers in Plant Science 12, 634023
Huebbers, JW., Buyel, JF. (2021) On the verge of the market - Plant factories for the automated and standardized production of biopharmaceuticals. Biotechnology Advances 46, 107681
Knödler, M., Buyel, JF. (2021) Plant-made immunotoxin building blocks: A roadmap for producing therapeutic antibody-toxin fusions. Biotechnology Advances 47, 107683
Nausch, H., Buyel, JF. (2021) Cryopreservation of plant cell cultures - Diverse practices and protocols. New Biotechnology 62, 86-95
Nordlund, E., Rischer, H. (2021): Plant cells grown in bioreactors. In: Cellular Agriculture - Lab-grown foods (Ercili-Cura, D. & Barth D. (Eds), American Chemical Society, doi: 10.1021/acs.infocus.7e4007)
Opdensteinen, P., Lobanov, A., Buyel, JF. (2021) A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins that are sensitive to extremes of either parameter. Biotechnology Journal 16, e2000340
Ponndorf D, Meshcheriakova Y, Thuenemann EC, Dobon Alonso A, Overman R, Holton N, Dowall S, Kennedy E, Stocks M, Lomonossoff GP, Peyret H. (2021) Plant-made dengue virus-like particles produced by co-expression of structural and non-structural proteins induce a humoral immune response in mice. Plant Biotechnology Journal 19, 745-756
Stanbekova, G., Beisenonov, D., Nizkorodova, A., Iskakov, B., Warzecha, H. (2021) Production of the sheep pox virus structural protein SPPV117 in tobacco chloroplasts. Biotechnolgy Letters, first published on 2/04/2021 (doi: 10.1007/s10529-021-03117-x)
Thuenemann, EC., Byrne, MJ., Peyret, H., Saunders, K., Castells-Graells, R., Ferriol, I., Santoni, M., Steele, JFC., Ranson, NA., Avesani, L., Lopez-Moya, JJ., Lomonossoff, GP. (2021) A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants. Viruses 13 885
Thuenemann, EC., Le, DHT., Lomonossoff, GP., Steinmetz, NF. (2021) Bluetongue Virus Particles as Nanoreactors for Enzyme Delivery and Cancer Therapy. Molecular Pharmaceutics 18, 1150-1156

2020

Bakshi, S., Sanz Garcia, R.°, Van der Weken, H., Tharad, A., Pandey, S., Juarez, P., Virdi, V., Devriendt, B., Cox, E., Depicker, A. (2020) Evaluating single-domain antibodies as carriers for targeted vaccine delivery to the small intestinal epithelium. Journal of Controlled Release 321, 416-429
Bayliss, M., Donaldson, M.I., Pergolizzi, G., Scott, A.E., Nepogodiev, S.A., Beales, L., Whelan, M., Rosenberg, W., Peyret, H., Lomonossoff, G.P., Harmer, N.J., Atkins, T., Field, R.., Prior, J.L. (2020) Assessments of hepatitis B virus-like particles and Crm197 as carrier proteins in melioidosis glycoconjugate vaccines. bioRxiv 2020.04.08.031658
Bernardi, A., Huang, Y., Xiong, Y., Nandi, S., McDonald, K.A., Faller, R. (2020) Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain. PLoS One 15, e0237295
Bohlender LL, Parsons J, Hoernstein SNW, Rempfer C, Ruiz-Molina N, Lorenz T, Rodríguez Jahnke F, Figl R, Fode B, Altmann F, Reski R, Decker EL. (2020) Stable Protein Sialylation in Physcomitrella. Froniers int Plant Science 11, 610032
Capell, T., Twyman, R.M., Armario-Najera, V., Ma, J.K.-C., Schillberg, S., Christou, P. (2020) Potential Applications of Plant Biotechnology against SARS-CoV-2. Trends in Plant Science 25, 635-643
Decker, EL., Reski, R. (2020) Mosses in biotechnology. Current Opinion in Biotechnology 61,21-27
De Greve, H., Vikram, V., Bakshi, S., Depicker, A. (2020) Simplified monomeric VHH-Fc antibodies provide new opportunities for passive immunization. Current Opinion in Biotechnololgy 61, 96-101
De Meyer, T., Arcalis, E., Melnik, S., Maleux, K., Nolf, J., Altmann, F., Depicker, A., Stöger, E. (2020). Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology. Plant Molecular Biology 103, 597-608
Fischer, R., Buyel, JF. (2020) Molecular farming - The slope of enlightenment. Biotechnology Advances 40, 107519
Gengenbach, BB., Opdensteinen, P., Buyel, JF. (2020) Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression. Frontiers in Bioengineering and Biotechnology 8, 393
Göritzer, K., Goet, I., Duric, S., Maresch, D., Altmann, F., Obinger, C., Strasser, R. (2020) Efficient N-Glycosylation of the Heavy Chain Tailpiece Promotes the Formation of Plant-Produced Dimeric IgA. Frontiers in Chemistry 8, 346
Gutierrez-Valdes N., Häkkinen S.T., Lemasson C., Guillet M., Oksman-Caldentey K.-M., Ritala A., Cardon F. (2020) Hairy root cultures – A versatile tool for multiple applications. Frontiers in Plant Science 11, 33
Häkkinen, S.T., Nygren, H., Nohynek, L., Puupponen-Pimiä, R., Heiniö, R.-L., Maiorova, N., Rischer, H., Ritala, R. (2020): Plant cells as food – aspects of sustainability and safety. Plant Cell Reports 39, 1655-1668
Jugler, C., Joensuu, J., Chen, Q. (2020) Hydrophobin-protein a fusion protein produced in plants efficiently purified an anti-west nile virus monoclonal antibody from plant extracts via aqueous two-phase separation. International Journal of Molecular Sciences 21, 2140
Ma, R., Yu, Z., Cai, Q., Li, H., Dong, Y., Oksman-Caldentey, KM., Rischer, H. (2020) Agrobacterium-Mediated Genetic Transformation of the Medicinal Plant Veratrum dahuricum. Plants 9, 191
Margolin, E., Oh, Y.J., Verbeek, M., Naude, K., Ponndorf, D., Meshcheriakova, Y.A., Peyret, H., van Diepen, M.T., Chapman, R., Meyers, A.E., Lomonossoff, G.P., Matoba, N., Williamson, A.-L., Rybicki, E.P. (2020) Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants. Plant Biotechnology Journal 18, 2109-2117
Margolin, E., Strasser R., Chapman, R., Williamson, A.-L., Rybicki, E.P., Meyers, A.E. (2020) Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals. Trends in Biotechnology 38, 1034-1044
Marques, LÉC., Silva, BB., Dutra, RF., Florean, EOPT., Menassa, R., Guedes, MIF. (2020) Transient Expression of Dengue Virus NS1 Antigen in Nicotiana benthamiana for Use as a Diagnostic Antigen. Frontiers in Plant Science 10, 1674
Menary, J., Hobbs, M., Mesquita de Albuquerque, S., Pacho, A., Drake, PMW., Prendiville, A., Ma, JK., Fuller, SS. (2020) Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products. PLoS ONE 15, e0229952
Murad, S., Fuller, S., Menary, J., Moore, C., Pinneh, E., Szeto, T., Hitzeroth, I., Freire, M., Taychakhoonavudh, S., Phoolcharoen, W., Ma, J.K.-C. (2020) Molecular Pharming for low and middle income countries. Current Opinion in Biotechnology 61, 53-59
Poborilova, Z., Plchova, H., Cerovska, N., Gunter, CJ., Hitzeroth, II., Rybicki, EP., Moravec, T. (2020) Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors. Plant Cell Reports 39, 1115-1127
Pihlajaniemi, V., Ellilä, S., Poikkimäki, S., Nappa, M., Rinne, M., Lantto, R., Siika-aho, M. (2020) Comparison of pretreatments and cost-optimization of enzymatic hydrolysis for production of single cell protein from grass silage fibre. Bioresource Technology Reports 9, 100357
Reski, R., Rybicki, E., Foster, G. (2020) Editorial overview: Plant biotechnology. Current Opinion in Biotechnology 61, iii-v
Rischer, H., Szilvay, GR., Oksman-Caldentey, KM. (2020) Cellular agriculture - industrial biotechnology for food and materials. Current Opinion in Biotechnology 61, 128-134
Ritala, A., Rischer, H., Häkkinen, S.T., Joensuu, J.J., Oksman-Caldentey, K.-M. (2020) Editorial: Proceedings of ISPMF 2018 - Plant Molecular Farming. Frontiers in Plant Science 11, 492
Schulman, AH., Oksman-Caldentey, KM., Teeri, TH. (2020) European Court of Justice delivers no justice to Europe on genome-edited crops. Plant Biotechnology Journal 18, 8-10
Stelter, S., Paul, M.J., Teh, A.Y.-H., Grandits, M., Altmann, F., Vanier, J., Bardor, M., Castilho, A., Allen, R.L., Ma, J.K.-C. (2020) Engineering the Interactions Between a Plant-Produced HIV Antibody and Human Fc Receptors. Plant Biotechnology Journal 18, 402-414
Tusé, D., Nandi, S., McDonald, KA., Buyel, JF. (2020) The Emergency Response Capacity of Plant-Based Biopharmaceutical Manufacturing-What It Is and What It Could Be. Frontiers in Plant Science 11, 594019
Velázquez-Lam, E., Imperial, J., Ponz, F. (2020) Polyphenol-Functionalized Plant Viral-Derived Nanoparticles Exhibit Strong Antimicrobial and Antibiofilm Formation Activities. ACS Applied Bio Materials 3, 2040-2047
van Noort, K., Nguyen, DL., Kriechbaumer, V., Hawes, C., Hokke, CH., Schots, A., Wilbers, RHP. (2020) Functional characterization of Schistosoma mansoni fucosyltransferases in Nicotiana benthamiana plants. Scientific Reports 10, 18528
Yuste-Calvo C, Ibort P, Sánchez F, Ponz F. (2020) Turnip Mosaic Virus Coat Protein Deletion Mutants Allow Defining Dispensable Protein Domains for 'in Planta' eVLP Formation. Viruses12, 661
Zampieri, R., Brozzetti, A.,Pericolini, E., Bartoloni, E., Gabrielli, E., Roselletti, E., Lomonosoff, G., Meshcheriakova, Y., Santi, L., Imperatori, F., Merlin, M., Tinazzi, E., Dotta, F., Nigi, L., Sebastiani, G., Pezzotti, M., Falorni, A., Avesani, L. (2020) Prevention and treatment of autoimmune diseases with plant virus nanoparticles. Science Advances 6, eaaz0295

2019

Akhgari, A., Laakso, I., Maaheimo, H., Choi, YH., Seppänen-Laakso, T., Oksman-Caldentey, KM., Rischer, H. (2019) Methyljasmonate Elicitation Increases Terpenoid Indole Alkaloid Accumulation in Rhazya stricta Hairy Root Cultures. Plants 8, 534
Alkanaimsh, S., Corbin, JM., Kailemia, M.J., Karuppanan, K., Rodriguez, RL., Lebrilla CB., McDonald, KA., Nandi, S. (2019). Purification and Site-specific N-glycosylation Analysis of Human Recombinant Butyrylcholinesterase from Nicotiana benthamiana. Biochemical Engineering Journal 142, 58-67
Arcalis, E., Ibl, I., Hilscher, J., Rademacher, T., Avesani, L., Morandini, F., Bortesi, L., Pezzotti, M., Vitale, A., Pum, D., De Meyer, T., Depicker, A., Stoger, E. (2019). Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production. Frontiers in Plant Science 10, 777
Bakshi, S., Depicker, A., Schepens, B., Saelens, X., Juarez, P. (2019) A two-amino acid mutation in murine IgA enables downstream processing and purification on staphylococcal superantigen-like protein 7. Journal of Biotechnology 294, 26-29
Burke, WG., Kaplanoglu, E., Kolotilin, I., Menassa, R., Donly, C. (2019) RNA Interference in the Tobacco Hornworm, <i>Manduca sexta</i>, Using Plastid-Encoded Long Double-Stranded RNA. Frontiers in Plant Science 10, 313
Buyel, JF. (2018) Plant Molecular Farming - Integration and Exploitation of Side Streams to Achieve Sustainable Biomanufacturing. Frontiers in Plant Science 9, 1893
Cardon, F., Pallisse, R., Bardor, M., Caron, A., Vanier, J., Ele Ekouna, JP., Lerouge, P., Boitel-Conti, M., Guillet, M. (2018) Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase. Plant Biotechnology Journal 17, 505-516
Cuesta R, Yuste-Calvo C, Gil-Cartón D, Sánchez F, Ponz F, Valle M. (2019) Structure of Turnip mosaic virus and its viral-like particles. Scienctific Reports 9, 15396
Gengenbach, BB., Keil, LL., Opdensteinen, P., Müschen, CR., Melmer, G., Lentzen, H., Bührmann, J., Buyel, JF. (2019) Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin. Biotechnology and Bioengeering 116, 2236-2249
Gunter, CJ., Regnard, GL., Rybicki, EP., Hitzeroth, II. (2019) Immunogenicity of plant-produced porcine circovirus-like particles in mice. Plant Biotechnology Journal 17, 1751-1759
Jansing, J., Buyel, JF. (2019) The correlation between DsRed mRNA levels and transient DsRed protein expression in plants depends on leaf age and the 5' untranslated region. Biotechnology Journal 14, 1800075
Jansing, J., Sack, M., Augustine, SM., Fischer, R., Bortesi, L. (2019) CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose. Plant Biotechnology Journal 17, 350-361
Knödler, M., Rühl, C., Emonts, J., Buyel, JF. (2019) Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting. Frontiers in Plant Science 10, 1245
Knödler, M., Rühl, C., Opdensteinen, P., Buyel, JF. (2019) Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. Journal of visualized experiments : JoVE 150, e59933
Kommineni, V., Markert, M., Ren, Z., Palle, S., Carrillo, B., Deng, J., Tejeda, A., Nandi, S., McDonald, KA., Marcel, S., Holtz, B. (2019). In Vivo Glycan Engineering via the Mannosidase I Inhibitor (Kifunensine) Improves Efficacy of Rituximab Manufactured in Nicotiana benthamiana Plants. International Journal of Molecular Sciences 20, 194
Kopertekh, L., Schiemann, J. (2019) Transient production of recombinant pharmaceutical proteins in plants: evolution and perspectives. Current Medicinal Chemistry 26, 365-380
Lonoce, C., Marusic, C., Morrocchi, E., Salzano, AM., Scaloni, A., Novelli, F., Pioli, C., Feeney, M., Frigerio, L., Donini, M. (2019) Enhancing the secretion of a glyco‐engineered anti‐CD20 scFv‐Fc antibody in hairy root cultures. Biotechnology Journal 14, 1800081
Mbewana, S., Meyers, AE., Rybicki, EP. (2019) Chimaeric Rift Valley fever virus-like particle vaccine candidate production in Nicotiana benthamiana. Biotechnology Journal 14, 1800238
McNulty, MJ., Gleba, Y., Tusé, D., Hahn-Löbmann, S., Giritch, A., Nandi, S., McDonald, KA. (2020) Techno-economic analysis of a plant-based platform for manufacturing antimicrobial proteins for food safety. Biotechnology Progress 36, e2896
Opdensteinen, P., Clodt, JI., Müschen, CR., Filiz, V., Buyel, JF. (2018) A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts. Frontiers in Bioengineering and Biotechnology 6, 206
Palaci, J., Virdi, V., Depicker, A. (2019) Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants. Plant Biotechnology Journal 17, 1760-1769
Rademacher, T., Sack, M., Blessing, D., Fischer, R., Holland, T., Buyel, J. (2019) Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering. Plant Biotechnology Journal 17, 1560-1566
Saberianfar, R., Chin-Fatt, A., Scott, A., Henry, KA., Topp, E., Menassa, R. (2019) Plant-Produced Chimeric VHH-sIgA Against Enterohemorrhagic E. coli Intimin Shows Cross-Serotype Inhibition of Bacterial Adhesion to Epithelial Cells. Frontiers in Plant Science 10, 270
Scott, IM., Zhu, H., Schieck, K., Follick, A., Reynolds, LB., Menassa, R. (2019) Non-target Effects of Hyperthermostable α-Amylase Transgenic Nicotiana tabacum in the Laboratory and the Field. Frontiers in Plant Science 10, 878
Spiegel, H., Boes, A., Perales Morales, C., Rademacher, T., Buyel, JF. (2019) Ready-to-Use Stocks of Agrobacterium tumefaciens Can Simplify Process Development for the Production of Recombinant Proteins by Transient Expression in Plants. Biotechnology Journal 14, e1900113
Top O, Parsons J, Bohlender LL, Michelfelder S, Kopp P, Busch-Steenberg C, Hoernstein SNW, Zipfel PF, Häffner K, Reski R, Decker EL. (2019) Recombinant Production of MFHR1, A Novel Synthetic Multitarget Complement Inhibitor, in Moss Bioreactors. Frontiers in Plant Science 10, 260
Top, O., Geisen, U., Decker, EL., Reski, R. (2019) Critical Evaluation of Strategies for the Production of Blood Coagulation Factors in Plant-Based Systems. Frontiers in Plant Science 10, 261
Tran, HH., Chen, B., Chen, H., Menassa, R., Hao, X., Bernards, M., Hüner, NPA., Wang, A. (2019) Development of a cucumber green mottle mosaic virus-based expression vector for the production in cucumber of neutralizing epitopes against a devastating animal virus. Journal of Virological Methods 269, 18-25
Virdi, V., Palaci, J., Laukens, B., Ryckaert, S., Cox, E., Vanderbeke, E., Depicker, A., Callewaert, N. (2019) Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model. Nature Biotechnology 37, 527-530
Xiong, Y., Karuppana, K., Bernardi, A., Li, Q., Kommineni, V., Dandekar, AM., Lebrilla, CB., Faller, R., McDonald, KA., Nandi, S. (2019). Effects of N-Glycosylation on the Structure, Function, and Stability of a Plant-Made Fc-Fusion Anthrax Decoy Protein. Frontiers in Plant Science 10, 768
Yuste-Calvo, C., González-Gamboa, I., Pacios, L.F., Sánchez, F., Ponz, F. (2019) Structure-Based Multifunctionalization of Flexuous Elongated Viral Nanoparticles. ACS Omega 4, 5019–5028
Yuste-Calvo C, López-Santalla M, Zurita L, Cruz-Fernández CF, Sánchez F, Garín MI, Ponz F. (2019) Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection. Nanomaterials 9, 1438

2018

Alam, A., Jiang, L., Kittleson, GA., Steadman, KD., Nandi, S., Fuqua, JL., Palmer, KE., Tusé, D., McDonald KA. (2018) Technoeconomic modeling of plant-based Griffithsin manufacturing. Frontiers in Bioengineering and Biotechnology 6, 102
Al-Handawi, MB., Commins, P., Shukla, S., Didier, P., Tanaka, M. Raj, G., Veliz, FA., Pasricha, R., Steinmetz, NF., Naumov, P. (2018) Encapsulation of plant viral particles in calcite crystals. Advanced Biosystems 2, 1700176
Amiri, M., Jalali-Javaran, M., Haddad, R., Ehsani, P. (2018) In silico and in vivo analyses of the mutated human tissue plasminogen activator (mtPA) and the antithetical effects of P19 silencing suppressor on its expression in two Nicotiana species. Scientific Reports 8, 14079
Appelhagen, I., Wulff-Vester, AK., Wendell, M., Hvoslef-Eide, AK., Russell, J., Oertel, A., Martens, S., Mock, HP., Martin, C., Matros, A. (2018) Colour bio-factories: towards scale-up production of anthocyanins in plant cell cultures. Metabolic Engineering 48, 218-232
Baudisch, B., Pfort, I., Sorge, E., Conrad, U. (2018) Nanobody-directed specific degradation of proteins by the 26S-proteasome in plants. Frontiers in Plant Science 9, 130
Berardi, A., Evans, DJ., Baldelli Bombelli, F., Lomonossoff, GP. (2018) Stability of plant virus-based nanocarriers in gastrointestinal fluids. Nanoscale 10, 1667-1679
Bruckman, MA., Czapar, AE., Steinmetz, NF. (2018) Drug-loaded plant-virus based nanoparticles for cancer drug delivery. Methods in Molecular Biology 1776, 425-436
Buyel, JF. (2018) Plants as sources of natural and recombinant anti-cancer agents. Biotechnology Advances 36, 506-520
Castells-Graells, R., Lomonossoff, GP., Saunders, K. (2018) Production of mosaic turnip crinkle virus-like particles derived by coinfiltration of wild-type and modified forms of virus coat protein in plants. Methods in Molecular Biology 1776, 3-17
Castilho, A., Beihammer, G., Pfeiffer, C., Göritzer, K., Montero‐Morales, L., Vavra, U., Maresch, D., Grünwald‐Gruber, C., Altmann, F., Steinkellner, H., Strasser, R. (2018) An oligosaccharyltransferase from Leishmania major increases the N‐glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana. Plant Biotechnology Journal 16, 1700-1709
Corbin, JM., Kailemia, MJ., Cadieux, CL., Alkanaimsh, S., Karuppanan, K., Rodriguez, RL., Lebrilla, CB., Cerasoli, DM., McDonald, KA., Nandi, S. (2018) Purification, characterization, and N‐glycosylation of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures. Biotechnology and Bioengineering 115, 1301-1310
Czapar, AE., Tiu, BDB., Veliz, FA., Pokorski, JK., Steinmetz, NF. (2018) Slow-release formulation of cowpea mosaic virus for in situ vaccine delivery to treat ovarian cancer. Advanced Science 5, 1700991
de Vera, CR., Díaz Crespín, G., Hernández Daranas, A., Montalvão Looga, S., Lillsunde, K-E., Tammela, P., Perälä, M., Hongisto, V., Virtanen, J., Rischer, H., Muller, CD., Norte, M., Fernández, JJ., Souto, ML. (2018) Marine microalgae: Promising source for new bioactive compounds. Marine Drugs 16, 317
Dennis, SJ., Meyers, AE., Guthrie, AJ., Hitzeroth, II., Rybicki, EP. (2018) Immunogenicity of plant-produced African horse sickness virus-like particles: implications for a novel vaccine. Plant Biotechnology Journal 16, 442-450
Dennis, SJ., O’Kennedy, MM., Rutkowska, D., Tsekoa, T., Lourens, CW., Hitzeroth, II., Meyers, AE., Rybicki, EP. (2018) Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses. Veterinary Research 49, 105
Dickmeis, C., Altintoprak, K., van Rijn, P., Wege, C., Commandeur, U. (2018) Bioinspired silica mineralization on viral templates. Methods in Molecular Biology 1776, 337-362
Eriksson, D., Brinch-Pedersen, H., Chawade, A., Holme, IB., Hvoslef-Eide, TAK., Ritala, A., Teeri, TH., Thorstensen, T. (2018) Scandinavian perspectives on plant gene technology: applications, policies and progress. Physiologia Plantarum 162, 219-238
Eriksson, D., De Andrade, E., Bohanec, B., Chatzopolou, S., Defez, R., Eriksson, NL., Van Der Meer, P., Van Der Meulen, B., Ritala, A., Sági, L., Schiemann, J., Twardowski, T., Vaněk, T. (2018) Why the European Union needs a national GMO opt-in mechanism. Nature Biotechnology 36, 18-19
Fischer, R., Holland, T., Sack, M., Schillberg, S., Stoger, E., Twyman, RM., Buyel, JF. (2018) Glyco-engineering of plant-based expression systems. Advances in Biochemical Engineering/Biotechnology, first published on 2/08/2018
Franke, CE., Czapar, AE., Patel, RB., Steinmetz, NF. (2018) Tobacco mosaic virus-delivered cisplatin restores efficacy in platinum-resistant ovarian cancer cells. Molecular Pharmaceutics 15,2922-2931
Gelová, Z., ten Hoopen, P., Novák, O., Motyka, V., Pernisová, M., Dabravolski, S., Didi, V., Tillack, I., Oklešťková, J., Strnad, M., Hause, B. (2018) Antibody-mediated modulation of cytokinins in tobacco: organ-specific changes in cytokinin homeostasis. Journal of Experimental Botany 69, 441-454
Gengenbach, BB., Müschen, CR., Buyel, JF. (2018) Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems. Protein Expression and Purification 151, 46-55
Gilchuk, P., Mire, CE., Geisbert, JB., Agans, KN., Deer, DJ., Cross, RW., Slaughter, JC., Flyak, AI., Mani, J., Pauly, MH., Velasco, J. (2018) Efficacy of human monoclonal antibody monotherapy against Bundibugyo virus infection in nonhuman primates. Journal of Infectious Diseases 218, S565-S573
Gulati, NM., Pitek, AS., Czapar, AE., Stewart, PL., Steinmetz, NF. (2018) The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition. Journal of Materials Chemistry B 6, 2204-2216
Gulati, NM., Stewart, PL., Steinmetz, NF. (2018) Bioinspired shielding strategies for nanoparticle drug delivery applications. Molecular Pharmaceutics 15, 2900-2909
Häkkinen, ST., Nuutila AM., Ritala, A. (2018) Modifying seeds to produce proteins. In: Proteins in Food Processing 2nd ed., R. Yada (Ed), Elsevier, San Diego USA, pp. 413-441
Häkkinen, ST., Nygren, H., Maiorova, N., Haavikko, R., Alakurtti, S., Yli-Kauhaluoma, J., Rischer, H., Oksman-Caldentey, KM. (2018) Biotransformation of cyclodextrine-complexed semisynthetic betulin derivatives by plant cells. Planta Medica 84, 743-748
Häkkinen, ST., Oksman-Caldentey, K-M. (2018) Progress and prospects of hairy root research. In: Hairy Roots - An Effective Tool of Plant Biotechnology, V. Srivastava, S. Mehrotra & S. Mishra (Eds), Springer, Singapore, pp. 3-19
Häkkinen, ST., Reuter, L., Nuorti, N., Joensuu, JJ., Rischer, H., Ritala, A. (2018) Tobacco BY-2 media component optimization for a cost-efficient recombinant protein production. Frontiers in Plant Science 9, 45
Henricks, LM., Siemerink, EJ., Rosing, H., Meijer, J., Goorden, SM., Polstra, AM., Zoetekouw, L., Cats, A., Schellens, JH., van Kuilenburg, AB. (2018) Capecitabine‐based treatment of a patient with a novel DPYD genotype and complete dihydropyrimidine dehydrogenase deficiency. International Journal of Cancer 142, 424-430
Hesketh, EL., Saunders, K., Fisher, C., Potze, J., Stanley, J., Lomonossoff, GP., Ranson, NA. (2018) The 3.3 Å structure of a plant geminivirus using cryo-EM. Nature Communications 9, 2369
Hitzeroth, II., Chabeda, A., Whitehead, MP., Graf, M., Rybicki, EP. (2018) Optimizing a human papillomavirus type 16 L1-based chimaeric gene for expression in plants. Frontiers in Bioengineering and Biotechnology 6, 101
Hoelscher, M., Tiller, N., Teh, AY., Wu, GZ., Ma, JK., Bock, R. (2018) High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves. Plant Molecular Biology 97, 357-370
Hoernstein, SN., Fode, B., Wiedemann, G., Lang, D., Niederkrüger, H., Berg, B., Schaaf, A., Frischmuth, T., Schlosser, A., Decker, EL., Reski, R. (2018) Host cell proteome of Physcomitrella patens harbors proteases and protease inhibitors under bioproduction conditions. Journal of Proteome Research 17, 3749-3760
Holland, T., Buyel, JF. (2018) Bioreactor-based production of glycoproteins in plant cell suspension cultures. Methods in Molecular Biology 1674, 129-146
Hoopes, PJ., Wagner, RJ., Duval, K., Kang, K., Gladstone, DJ., Moodie, KL., Crary-Burney, M., Ariaspulido, H., Veliz, F.A., Steinmetz, NF. and Fiering, SN. (2018) Treatment of canine oral melanoma with nanotechnology-based immunotherapy and radiation. Molecular Pharmaceutics 15, 3717-3722
Huang, TK., Falk, BW., Dandekar, AM., McDonald, KA. (2018) Enhancement of recombinant protein production in transgenic Nicotiana benthamiana plant cell suspension cultures with co-cultivation of Agrobacterium containing silencing suppressors. International Journal of Molecular Sciences 19, 1561
Huddy, SM., Hitzeroth, II., Meyers, AE., Weber, B., Rybicki, EP. (2018) Transient expression and purification of horseradish peroxidase C in Nicotiana benthamiana. International Journal of Molecular Sciences 19, 115
Jackson, MA., Gilding, EK., Shafee, T., Harris, KS., Kaas, Q., Poon, S., Yap, K., Jia, H., Guarino, R., Chan, LY., Durek, T. (2018) Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases. Nature Communications 9, 2411
Jutras, PV., Goulet, MC., Lavoie, PO., D'Aoust, MA., Sainsbury, F., Michaud, D. (2018) Recombinant protein susceptibility to proteolysis in the plant cell secretory pathway is pH-dependent. Plant Biotechnology Journal 16, 1928-1938
Kim, MY., Copland, A., Nayak, K., Chandele, A., Ahmed, MS., Zhang, Q., Diogo, GR., Paul, MJ., Hofmann, S., Yang, MS. and Jang, YS. (2018) Plant‐expressed Fc‐fusion protein tetravalent dengue vaccine with inherent adjuvant properties. Plant Biotechnology Journal 16, 1283-1294
Koistinen, VM, da Silva, AB., Abrankó, L., Low, D., Villalba, RC., Tomás Barberán, F., Landberg, R., Savolainen, O., Alvarez-Acero, I., de Pascual-Teresa, S., Van Poucke, C., Almeida, C., Petrásková, L., Valentová, K., Durand, S., Wiczkowski, W., Szawara-Nowak, D., González-Domínquez, R., Llorach, R., Andrés-Lucueva, C., Aura, A-M., Seppänen-Laakso, T., Hanhineva, K., Manach, C., Bronze, MR. (2018) Interlaboratory coverage test on plant food bioactive compounds and their metabolites by mass spectrometry-based untargeted metabolomics. Metabolites 8, 46
Kurppa, K., Reuter, LJ., Ritala, A., Linder, MB., Joensuu, JJ. (2018) In-solution antibody harvesting with a plant-produced hydrophobin-Protein A fusion. Plant Biotechnology Journal 16, 404-414
Kytidou, K., Beekwilder, J., Artola, M., van Meel, E., Wilbers, RH., Moolenaar, GF., Goosen, N., Ferraz, MJ., Katzy, R., Voskamp, P., Florea, BI. (2018) Nicotiana benthamiana α-galactosidase A1. 1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease. Journal of Biological Chemistry 293, 10042-10058
Lam, P., Lin RD., Steinmetz, NF. (2018) Delivery of mitoxantrone using a plant virus-based nanoparticle for the treatment of glioblastomas. Journal of Materials Chemistry B 6, 5888-5895
Lam, P., Steinmetz, NF. (2018) Plant viral and bacteriophage delivery of nucleic acid therapeutics. Nanomedicine and Nanobiotechnology 10, e1487
Lin, RD., Steinmetz, NF. (2018) Tobacco mosaic virus delivery of mitoxantrone for cancer therapy. Nanoscale 10, 16307-16313
Lomonossoff, GP. (2018) So what have plant viruses ever done for virology and molecular biology? Advances in Virus Research 100, 145-162
Lomonossoff, GP., Wege, C. (2018) TMV particles: the journey from fundamental studies to bionanotechnology applications. Advances in Virus Research 102, 149-176
Margolin, E., Chapman, R., Williamson, AL., Rybicki, EP., Meyers, AE. (2018) Production of complex viral glycoproteins in plants as vaccine immunogens. Plant Biotechnology Journal 16, 1531-1545
Marjamaa, K., Kruus, K. (2018) Enzyme biotechnology in degradation and modification of plant cell wall polymers. Physiologia Plantarum 164, 106-118
Marusic, C., Pioli, C., Stelter, S., Novelli, F., Lonoce, C., Morrocchi, E., Benvenuto, E., Salzano, AM., Scaloni, A., Donini, M. (2018) N-glycan engineering of a plant-produced anti-CD20-hIL-2 immunocytokine significantly enhances its effector functions. Biotechnology and Bioengineering 115, 565-576
Menzel, S., Holland, T., Boes, A., Spiegel, H., Fischer, R., Buyel, JF. (2018) Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate. Protein Expression and Purification 152, 122-130
Michelfelder, S., Fischer, F., Wäldin, A., Hörle, K.V., Pohl, M., Parsons, J., Reski, R., Decker, EL., Zipfel, PF., Skerka, C., Häffner, K. (2018) The MFHR1 fusion protein is a novel synthetic multitarget complement inhibitor with therapeutic potential. Journal of the American Society of Nephrology 29, 1141-1153
Murray, AA., Wang, C., Fiering, S., Steinmetz, NF. (2018) In situ vaccination with cowpea vs tobacco mosaic virus against melanoma. Molecular Pharmaceutics 15, 3700-3716
Nordlund, E., Lille, M., Silventoinen, PC., Nygren, H., Seppänen-Laakso, T., Mikkelsson, A., Aura, A.-M., Heiniö, R-L., Nohynek, L., Puupponen-Pimiä, R., Rischer, H. (2018) Plant cells as food - A concept taking shape. Food Research International 107, 297-305
Patel, R., Czapar, AE., Fiering, S., Oleinick, NL., Steinmetz, NF. (2018) Radiation therapy combined with cowpea mosaic virus nanoparticle in situ vaccination initiates immune-mediated tumor regression. ACS Omega 3, 3702-3707
Phan, HT., Gresch, U., Conrad, U. (2018) In vitro-formulated oligomers of strep-tagged avian influenza haemagglutinin produced in plants cause neutralizing immune responses. Frontiers in Bioengineering and Biotechnology 6, 115
Phoolcharoen, W., Banyard, AC., Prehaud, C., Selden, D., Wu, G., Birch, CP., Szeto, TH., Lafon, M., Fooks, AR., Ma, JKC. (2018) In vitro and in vivo evaluation of a single chain antibody fragment generated in planta with potent rabies neutralisation activity. Vaccine, first published on 6/03/2018
Pitek, AS., Park, J., Wang, Y., Gao, H., Hu, H., Simon, DI., Steinmetz, NF. (2018) Delivery of thrombolytic therapy using rod-shaped plant viral nanoparticles decreases the risk of hemorrhage. Nanoscale 10, 16547-16555
Pitek, AS., Veliz, FA., Jameson, SA., Steinmetz, NF. (2018) Interactions between plant viral nanoparticles (VNPs) and blood plasma proteins, and their impact on the VNP in vivo fates. Methods in Molecular Biology 1776, 591-608
Ramírez-Alanis, IA., Renaud, JB., García-Lara, S., Menassa, R., Cardineau, GA. (2018) Transient co-expression with three O-glycosylation enzymes allows production of GalNAc-O-glycosylated Granulocyte-Colony Stimulating Factor in N. benthamiana. Plant Methods 14, 98
Reski, R., Bae, H., Simonsen, HT. (2018) Physcomitrella patens, a versatile synthetic biology chassis. Plant Cell Reports 37, 1409-1417
Röder, J., Dickmeis, C., Fischer, R., Commandeur, U. (2018) Systemic infection of Nicotiana benthamiana with Potato virus X nanoparticles presenting a fluorescent iLOV polypeptide fused directly to the coat protein. BioMed Research International 2018,9328671
Röder, J., Dickmeis, C., Fischer, R., Commandeur, U. (2018) Systemic infection of Nicotiana benthamiana with Potato virus X nanoparticles presenting a fluorescent iLOV polypeptide fused directly to the coat protein. BioMed Research International 2018, 9328671
Rühl, C., Knödler, M., Opdensteinen, P., Buyel, JF. (2018) A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies. Journal of Chromatography A 1571, 55-64
Ruiz, V., Baztarrica, J., Rybicki, EP., Meyers, AE., Wigdorovitz, A. (2018) Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice. Biotechnology Reports 20, e00283
Sánchez, F., Ponz, F. (2018) Presenting peptides at the surface of potyviruses in planta. Methods in Molecular Biology 1776, 471-485
Santos, RB., Chandrasekar, B., Mandal, MK., Kaschani, F., Kaiser, M., Both, L., van der Hoorn, RA., Schiermeyer, A., Abranches, R. (2018) Low protease content in Medicago truncatula cell cultures facilitates recombinant protein production. Biotechnology Journal 13, 1800050
Schaaf, A., Tschogonov, T., Michelfelder, S., Krieghoff, N., Koch, J., Busch, A., Dabrowska-Schlepp, P., Bohlender, L., Parsons, J., Decker, EL., Reski, R., Haeffner, K., Frischmuth, T. (2018) Moss-made complement therapeutics. Molecular Immunology 102, 209
Shukla, S., Dickmeis, C., Fischer, R., Commandeur, U., Steinmetz, NF. (2018) In planta production of fluorescent filamentous plant virus-based nanoparticles. Methods in Molecular Biology 1776, 61-84
Silberstein, DZ., Karuppanan, K., Aung, HH., Chen, CH., Cross, CE., McDonald, KA. (2018) An oxidation-resistant, recombinant alpha-1 antitrypsin produced in Nicotiana benthamiana. Free Radical Biology & Medicine 120, 303-310
Sim, JS., Kesawat, MS., Kumar, M., Kim, SY., Mani, V., Subramanian, P., Park, S., Lee, CM., Kim, SR. and Hahn, BS. (2018) Lack of the α1,3-fucosyltransferase gene (Osfuct) affects anther development and pollen viability in rice. International Journal of Molecular Sciences 19, 1225
Sukenik, SC., Karuppanan, K., Li, Q., Lebrilla, CB., Nandi, S., McDonald, KA. (2018) Transient recombinant protein production in glycoengineered Nicotiana benthamiana cell suspension culture. International Journal of Molecular Sciences 19, 1205
Thuenemann, EC., Lomonossoff, GP. (2018) Delivering cargo: plant-based production of bluetongue virus core-like and virus-like particles containing fluorescent proteins. Methods in Molecular Biology 1776, 319-334
Tian, Y., Liimatainen, J., Puganen, A., Alakomi, HL., Sinkkonen, J., Yang, B. (2018) Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants. Food Research International 113, 115-130
Tian, Y., Puganen, A., Alakomi, HL., Uusitupa, A., Saarela, M., Yang, B. (2018) Antioxidative and antibacterial activities of aqueous ethanol extracts of berries, leaves, and branches of berry plants. Food Research International 106, 291-303
Tiu, BDB., Advincula, RC., Steinmetz, NF. (2018) Nanomanufacture of free-standing, porous, janus-type films of polymer-plant virus nanoparticle arrays. Methods in Molecular Biology 1776, 143-157
Vanier, G., Stelter, S., Vanier, J., Hempel, F., Maier, U. G., Lerouge, P., Ma, J., Bardor, M. (2018) Alga‐made anti‐hepatitis B antibody binds to human Fcγ receptors. Biotechnology Journal 13, 1700496
Vanmarsenille, C., Elseviers, J., Yvanoff, C., Hassanzadeh-Ghassabeh, G., Garcia Rodriguez, G., Martens, E., Depicker, A., Martel, A., Haesebrouck, F., Pasmans, F., Hernalsteens, JP., De Greve, H. (2018) In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter. PLoS One 13, e0204222
Veerapen, VP., van Zyl, AR., Rybicki, EP., Meyers, AE. (2018) Transient expression of heat- and acid-resistant foot-and-mouth disease virus P1-2A mutants in Nicotiana benthamiana. Virus Research 256, 45-49
Veerapen, VP., van Zyl, AR., Wigdorovitz, A., Rybicki, EP., Meyers, AE. (2018) Novel expression of immunogenic foot-and-mouth disease virus-like particles in Nicotiana benthamiana. Virus Research 244, 213-217
Vernekar, AA., Berger, G., Czapar, AE., Veliz, FA., Wang, DI., Steinmetz, NF., Lippard, SJ. (2018) Speciation of phenanthriplatin and its analogs in the core of tobacco mosaic virus. Journal of the American Chemical Society 140, 4279-4287
Wang, Y., Seppänen-Laakso, T., Rischer, H., Wiebe, MG. (2018) Euglena gracilis growth and cell composition under different temperature, light and trophic conditions. PLoS ONE 13, e0195329
Webster, GR., van Dolleweerd, C., Guerra, T., Stelter, S., Hofmann, S., Kim, MY., Teh, AYH., Diogo, GR., Copland, A., Paul, M., Hart, P. (2018) A polymeric immunoglobulin‐antigen fusion protein strategy for enhancing vaccine immunogenicity. Plant Biotechnology Journal 16, 1983-1996
Weerawanich, K., Webster, G., Ma, JK., Phoolcharoen, W., Sirikantaramas, S. (2018) Gene expression analysis, subcellular localization, and in planta antimicrobial activity of rice (Oryza sativa L.) defensin 7 and 8. Plant Physiology and Biochemistry 124, 160-166
Xiong, Y., Li, Q., Kailemia, MJ., Lebrilla, CB., Nandi, S., McDonald, KA. (2018) Glycoform modification of secreted recombinant glycoproteins through kifunensine addition during transient vacuum agroinfiltration. International Journal of Molecular Sciences 19, 890
Yanez, RJR., Lamprecht, R., Granadillo, M., Torrens, I., Arcalís, E., Stöger, E., Rybicki, EP., Hitzeroth, II. (2018) LALF32-51-E7, a HPV-16 therapeutic vaccine candidate, forms protein body-like structures when expressed in Nicotiana benthamiana leaves. Plant Biotechnology Journal 16, 628-637
Zheng, Y., Dougherty, ML., Konkolewicz, D., Steinmetz, NF., Pokorski, JK. (2018) Green nanofillers: plant virus reinforcement in hydrophilic polymer nanocomposites. Polymer 142, 72-79

2017

Akhgari, A., Oksman-Caldentey, KM., Rischer, H. (2017) Biotechnology of the medicinal plant Rhazya stricta: a little investigated member of the Apocynaceae family. Biotechnology Letters 39, 829-840
Anderson, CE., Donnola, SB., Jiang, Y., Batesole, J., Darrah, R., Drumm, ML., Brady-Kalnay, SM., Steinmetz, NF., Yu, X., Griswold, MA., Flask, CA. (2017) Dual contrast-magnetic resonance fingerprinting (DC-MRF): a platform for simultaneous quantification of multiple MRI contrast agents. Scientific Reports 7, 8431
Berardi, A., Lomonossoff, GP., Evans, DJ., Barker, SA. (2017) Plant-expressed hepatitis B core antigen virus-like particles: characterization and investigation of their stability in simulated and pig gastro-intestinal fluids. International Journal of Pharmaceutics 522, 147-156
Bludau, H., Czapar, AE., Pitek, AS., Shukla, S., Jordan, R., Steinmetz, NF. (2017) POxylation as an alternative stealth coating for biomedical applications. European Polymer Journal 88, 679-688
Buyel, JF., Gruchow, HM., Wehner, M. (2017) A rapid laser probing method facilitates the non-invasive and contact-free determination of leaf thermal properties. Journal of Visualized Experiments 2017 (119), 54835
Buyel, JF., Twyman, RM., Fischer, R. (2017) Very-large-scale production of antibodies in plants: the biologization of manufacturing. Biotechnology Advances 35, 458-465
Ceballo, Y., Tiel, K., López, A., Cabrera, G., Pérez, M., Ramos, O., Rosabal, Y., Montero, C., Menassa, R., Depicker, A., Hernández, A. (2017) High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza. Transgenic Research 26, 775-789
Chariou, PL., Steinmetz, NF. (2017) Delivery of pesticides to plant parasitic nematodes using tobacco mild green mosaic virus as a nanocarrier. ACS Nano 11, 4719-4730
Cho, CF., Yu, L., Nsiama, TK., Kadam, AN., Raturi, A., Shukla, S., Amadei, GA., Steinmetz, NF., Luyt, LG., Lewis, JD. (2017) Viral nanoparticles decorated with novel EGFL7 ligands enable intravital imaging of tumor neovasculature. Nanoscale 9, 12096-12109
Czapar, AE., Steinmetz, NF. (2017) Plant viruses and bacteriophages for drug delivery in medicine and biotechnology. Current Opinion in Chemical Biology 38, 108-116
Dehghan, E., Reed, DW., Covello, PS., Hasanpour, Z., Palazon, J., Oksman-Caldentey, KM., Ahmadi, FS. (2017) Genetically engineered hairy root cultures of Hyoscyamus senecionis and H. muticus: ploidy as a promising parameter in the metabolic engineering of tropane alkaloids. Plant Cell Reports 36, 1615-1626
Drake, PM., Szeto, TH., Paul, MJ., Teh, AY., Ma, JK. (2017) Recombinant biologic products versus nutraceuticals from plants - a regulatory choice? British Journal of Clinical Pharmacology 83, 82-87
Edgue, G., Twyman, RM., Beiss, V., Fischer, R., Sack, M. (2017) Antibodies from plants for bionanomaterials. Wiley interdisciplinary reviews. Nanomedicine and Nanobiotechnology 9, e1462
González-Gamboa, I., Manrique, P., Sánchez, F., Ponz, F. (2017) Plant-made potyvirus-like particles used for log-increasing antibody sensing capacity. Journal of Biotechnology 254, 17-24
Gulati, NM., Pitek, AS., Steinmetz, NF., Stewart, PL. (2017) Cryo-electron tomography investigation of serum albumin-camouflaged tobacco mosaic virus nanoparticles. Nanoscale 9, 3408-3415
Häkkinen, ST., Seppänen-Laakso, T., Oksman-Caldentey, K-M., Rischer, H. (2017) Bioconversion to raspberry ketone is achieved by several non-related plant cell cultures. In: Engineering the Plant Factory for the Production of Biologics and Small-Molecule Medicines, D. De Martinis, R. Franconi, E. Benvenuto, EP. Rybicki & K. Fujiyama (Eds), Frontiers Media, Lausanne, pp. 334-342
Havenith, H., Kern, K., Rautenberger, P., Spiegel, H., Szardenings, M., Ueberham, E., Lehmann, J., Buntru, M., Vogel, S., Treudler, R., Fischer, R. (2017) Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnology Journal 12, 1600441
Hesketh, EL., Meshcheriakova, Y., Thompson, RF., Lomonossoff, GP., Ranson, NA. (2017) The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Scientific Reports 7, 539
Horn, P., Nausch, H., Baars, S., Schmidtke, J., Schmidt, K., Schneider, A., Leister, D., Broer, I. (2017) Paternal inheritance of plastid-encoded transgenes in Petunia hybrida in the greenhouse and under field conditions. Biotechnology Reports 16, 26-31
Horn, P., Schlichting, A., Baum, C., Hammesfahr, U., Thiele-Bruhn, S., Leinweber, P., Broer, I. (2017) Fast and sensitive in vivo studies under controlled environmental conditions to substitute long-term field trials with genetically modified plants. Journal of Biotechnology 243, 48-60
Horn, P., Schlichting, A., Baum, C., Hammesfahr, U., Thiele-Bruhn, S., Leinweber, P., Broer, I. (2017) Reprint of “fast and sensitive in vivo studies under controlled environmental conditions to substitute long-term field trials with genetically modified plants”. Journal of Biotechnology 257, 22-34
Hotti, H., Gopalacharyulu, P., Seppänen-Laakso, T., Rischer, H. (2017) Metabolite profiling of the carnivorous pitcher plants Darlingtonia and Sarracenia. PLoS ONE 12, e0171078
Hotti, H., Häkkinen, ST., Seppänen-Laakso, T., Rischer, H. (2017) Polyketide-derived alkaloids and anthraquinones in Aloe plants and cell cultures. Journal of Plant Biotechnololgy Research 1, 1-15
Hotti, H., Rischer, H. (2017) The killer of Socrates: coniine and related alkaloids in the plant kingdom. Molecules 22, 1962
Houdelet, M., Galinski, A., Holland, T., Wenzel, K., Schillberg, S., Buyel, JF. (2017) Animal component‐free Agrobacterium tumefaciens cultivation media for better GMP‐compliance increases biomass yield and pharmaceutical protein expression in Nicotiana benthamiana. Biotechnology Journal 12, 1600721
Hu, H., Zhang, Y., Shukla, S., Gu, Y., Yu, X., Steinmetz, NF. (2017) Dysprosium-modified tobacco mosaic virus nanoparticles for ultra-high-field magnetic resonance and near-infrared fluorescence imaging of prostate cancer. ACS Nano 11, 9249-9258
Karuppanan, K., Duhra-Gill, S., Kailemia, MJ., Phu, ML., Lebrilla, CB., Dandekar, AM., Rodriguez, RL., Nandi, S., McDonald, KA. (2017) Expression, purification, and biophysical characterization of a secreted anthrax decoy fusion protein in Nicotiana benthamiana. International Journal of Molecular Sciences 18, 89
Kernan, DL., Wen, AM., Pitek, AS., Steinmetz, NF. (2017) Featured Article: Delivery of chemotherapeutic vcMMAE using tobacco mosaic virus nanoparticles. Experimental Biology and Medicine 242, 1405-1411
Kim, MY., Van Dolleweerd, C., Copland, A., Paul, MJ., Hofmann, S., Webster, GR., Julik, E., Ceballos‐Olvera, I., Reyes‐del Valle, J., Yang, MS., Jang, YS. (2017) Molecular engineering and plant expression of an immunoglobulin heavy chain scaffold for delivery of a dengue vaccine candidate. Plant Biotechnology Journal 15, 1590-1601
Kopertekh, L., Schiemann, J. (2019) Transient production of recombinant pharmaceutical proteins in plants: evolution and perspectives. Current Medicinal Chemistry 26, 365-380
Lam P., Keri R.A., Steinmetz N.F. (2017) A bioengineered positive control for rapid detection of the Ebola virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP). ACS Biomaterials Science and Engineering, 3, 452-459
Lauria, I., Dickmeis, C., Röder, J., Beckers, M., Rütten, S., Lin, YY., Commandeur, U., Fischer, H. (2017) Engineered potato virus X nanoparticles support hydroxyapatite nucleation for improved bone tissue replacement. Acta Biomaterialia 62, 317-327
Le, DH., Lee, KL., Shukla, S., Commandeur, U., Steinmetz, NF. (2017) Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy. Nanoscale 9, 2348-2357
Le, DHT., Hu, H., Commandeur, U., Steinmetz, NF. (2017) Chemical addressability of potato virus X for its applications in bio/nanotechnology. Journal of Structural Biology 200, 360-368
Lee, KL., Murray, AA., Le, DH., Sheen, MR., Shukla, S., Commandeur, U., Fiering, S., Steinmetz, NF. (2017) Combination of plant virus nanoparticle-based in situ vaccination with chemotherapy potentiates antitumor response. Nano Letters 17, 4019-4028
Lee, PW., Isarov, SA., Wallat, JD., Molugu, SK., Shukla, S., Sun, JE., Zhang, J., Zheng, Y., Lucius Dougherty, M., Konkolewicz, D., Stewart, PL. (2017) Polymer structure and conformation alter the antigenicity of virus-like particle–polymer conjugates. Journal of the American Chemical Society 139, 3312-3315
Lee, PW., Shukla, S., Wallat, JD., Danda, C., Steinmetz, NF., Maia, J., Pokorski, JK. (2017) Biodegradable viral nanoparticle/polymer implants prepared via melt-processing. ACS Nano 11, 8777-8789
Leth, IK., McDonald, KA. (2017) Growth kinetics and scale-up of Agrobacterium tumefaciens. Applied Microbiology and Biotechnology 101, 4895-4903
Leth, IK., McDonald, KA. (2017) Media development for large scale Agrobacterium tumefaciens culture. Biotechnology Progress 33, 1218-1225
MacDonald, J., Miletic, S., Gaildry, T., Chin-Fatt, A., Menassa, R. (2017) Co-expression with the type 3 secretion chaperone CesT from enterohemorrhagic E. coli increases accumulation of recombinant Tir in plant chloroplasts. Frontiers in Plant Science 8, 283
Mardanova, ES., Blokhina, EA., Tsybalova, LM., Peyret, H., Lomonossoff, GP., Ravin, NV. (2017) Efficient transient expression of recombinant proteins in plants by the novel pEff vector based on the genome of potato virus X. Frontiers in Plant Science 8, 247
Marsian, J., Fox, H., Bahar, MW., Kotecha, A., Fry, EE., Stuart, DI., Macadam, AJ., Rowlands, DJ., Lomonossoff, GP. (2017) Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine. Nature Communications 8, 245
Masarapu, H., Patel, BK., Chariou, PL., Hu, H., Gulati, NM., Carpenter, BL., Ghiladi, RA., Shukla, S., Steinmetz, NF. (2017) Physalis mottle virus-like particles as nanocarriers for imaging reagents and drugs. Biomacromolecules 18, 4141-4153
Matías‐Hernández, L., Jiang, W., Yang, K., Tang, K., Brodelius, PE., Pelaz, S. (2017) AaMYB 1 and its orthologue At MYB 61 affect terpene metabolism and trichome development in Artemisia annua and Arabidopsis thaliana. Plant Journal 90, 520-534
Mazalovska, M., Varadinov, N., Koynarski, T., Minkov, I., Teoharov, P., Lomonossoff, GP., Zahmanova, G. (2017) Detection of serum antibodies to hepatitis E virus based on HEV genotype 3 ORF2 capsid protein expressed in Nicotiana benthamiana. Annals of Laboratory Medicine 37, 313-319
Meshcheriakova, Y., Durrant, A., Hesketh, EL., Ranson, NA., Lomonossoff, GP. (2017) Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. Biochemical Society Transactions 45, 1263-1269
Michelfelder, S., Parsons, J., Bohlender, LL., Hoernstein, SN., Niederkrüger, H., Busch, A., Krieghoff, N., Koch, J., Fode, B., Schaaf, A., Frischmuth, T. (2017) Moss-produced, glycosylation-optimized human factor H for therapeutic application in complement disorders. Journal of the American Society of Nephrology 28, 1462-1474
Miletic, S., Hünerberg, M., Kaldis, A., MacDonald, J., Leuthreau, A., McAllister, T., Menassa, R. (2017) A plant‐produced candidate subunit vaccine reduces shedding of enterohemorrhagic Escherichia coli in ruminants. Biotechnology Journal 12, 1700405
Mire, CE., Geisbert, JB., Borisevich, V., Fenton, KA., Agans, KN., Flyak, AI., Deer, DJ., Steinkellner, H., Bohorov, O., Bohorova, N., Goodman, C. (2017) Therapeutic treatment of Marburg and Ravn virus infection in nonhuman primates with a human monoclonal antibody. Science Translational Medicine 9, eaai8711
Mohammadzadeh S. , Rahimi S. , Ebrahimi-Rad M., Ofoghi H. and Ehsani P. (2017) Transient expression of virus-like particles in plants: a promising platform for rapid vaccine production. Vaccine Research 4, 72-80
Nausch, H., Broer, I. (2017) Cyanophycinase CphE from P. alcaligenes produced in different compartments of N. benthamiana degrades high amounts of cyanophycin in plant extracts. Applied Microbiology and Biotechnology 101, 2397-2413
Pham, NB., Ho, TT., Nguyen, GT., Le, TT., Le, NT., Chang, HC., Pham, MD., Conrad, U., Chu, HH. (2017) Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein. Journal of Nanobiotechnology 15, 69
Phan, HT., Ho, TT., Chu, HH., Vu, TH., Gresch, U., Conrad, U. (2017) Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants. Veterinary Research 48, 53
Phoolcharoen, W., Prehaud, C., van Dolleweerd, CJ., Both, L., da Costa, A., Lafon, M., Ma, JK. (2017) Enhanced transport of plant-produced rabies single-chain antibody-RVG peptide fusion protein across an in cellulo blood-brain barrier device. Plant Biotechnology Journal 15, 1331-1339
Ponndorf, D., Broer, I., Nausch, H. (2017) Expression of CphB- and CphE-type cyanophycinases in cyanophycin-producing tobacco and comparison of their ability to degrade cyanophycin in plant and plant extracts. Transgenic Research 26, 491-499
Ponndorf, D., Ehmke, S., Walliser, B., Thoss, K., Unger, C., Görs, S., Daş, G., Metges, CC., Broer, I., Nausch, H. (2017) Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine. Plant Biotechnology Journal 15, 605-613
Poudel, L., Twarock, R., Steinmetz, NF., Podgornik, R., Ching, WY. (2017) Impact of hydrogen bonding in the binding site between capsid protein and MS2 bacteriophage ssRNA. Journal of Physical Chemistry B 121, 6321-6330
Rattanapisit, K., Abdulheem, S., Chaikeawkaew, D., Kubera, A., Mason, HS., Ma, JK., Pavasant, P., Phoolcharoen, W. (2017) Recombinant human osteopontin expressed in Nicotiana benthamiana stimulates osteogenesis related genes in human periodontal ligament cells. Scientific Reports 7, 17358
Regnard, GL., de Moor, WRJ., Hitzeroth, II., Williamson, AL., Rybicki, EP. (2017) Xenogenic rolling-circle replication of a synthetic beak and feather disease virus genomic clone in 293TT mammalian cells and Nicotiana benthamiana. Journal of General Virology 98, 2329-2338
Regnard, GL., Rybicki, EP., Hitzeroth, II. (2017) Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana. Virology Journal 14, 174
Reuter, LJ., Shahbazi, M-A., Mäkilä, EM., Salonen, JJ., Saberianfar, R., Menassa, R., Santos, HA., Joensuu, JJ., Ritala, A. (2017) Coating nanoparticles with plant-produced transferrin-hydrophobin fusion protein enhances their uptake in cancer cells. Bioconjugate Chemistry 28, 1639-1648
Reuter, LJ., Shahbazi, MA., Makila, EM., Salonen, JJ., Saberianfar, R., Menassa, R., Santos, HA., Joensuu, JJ., Ritala, A. (2017) Coating nanoparticles with plant-produced transferrin–hydrophobin fusion protein enhances their uptake in cancer cells. Bioconjugate Chemistry, 28, 1639-1648
Rybicki, EP. (2017) Plant-made vaccines and reagents for the One Health initiative. Human Vaccines & Immunotherapeutics 13, 2912-2917
Saberianfar, R., Menassa, R. (2017) Protein bodies: how the ER deals with high accumulation of recombinant proteins. Plant Biotechnology Journal 15, 671-673
Sandmann, M., Talbert, P., Demidov, D., Kuhlmann, M., Rutten, T., Conrad, U., Lermontova, I. (2017) Targeting of A. thaliana KNL2 to centromeres depends on the conserved CENPC-k motif in its C-terminus. Plant Cell 29, 144-155
Saunders, K., Lomonossoff, GP. (2017) In planta synthesis of designer-length tobacco mosaic virus-based nano-rods that can be used to fabricate nano-wires. Frontiers in Plant Science 8, 1335
Schmidt, K., Schmidtke, J., Mast, Y., Waldvogel, E., Wohlleben, W., Klemke, F., Lockau, W., Hausmann, T., Hühns, M., Broer, I. (2017) Comparative statistical component analysis of transgenic, cyanophycin-producing potatoes in greenhouse and field trials. Transgenic Research 26, 529-539
Shin, YJ., Castilho, A., Dicker, M., Sádio, F., Vavra, U., Grünwald‐Gruber, C., Kwon, TH., Altmann, F., Steinkellner, H., Strasser, R. (2017) Reduced paucimannosidic N‐glycan formation by suppression of a specific β‐hexosaminidase from Nicotiana benthamiana. Plant Biotechnology Journal 15, 197-206
Shukla, S., Myers, JT., Woods, SE., Gong, X., Czapar, AE., Commandeur, U., Huang, AY., Levine, AD., Steinmetz, NF. (2017) Plant viral nanoparticles-based HER2 vaccine: Immune response influenced by differential transport, localization and cellular interactions of particulate carriers. Biomaterials 121, 15-27
Spiegel, H., Boes, A., Fendel, R., Reimann, A., Schillberg, S., Fischer, R. (2017) Immunization with the malaria diversity-covering blood-stage vaccine candidate Plasmodium falciparum apical membrane antigen 1 DiCo in complex with its natural ligand PfRon2 does not improve the in vitro efficacy. Frontiers in Immunology 8, 743
Steele, JF., Peyret, H., Saunders, K., Castells‐Graells, R., Marsian, J., Meshcheriakova, Y., Lomonossoff, GP. (2017) Synthetic plant virology for nanobiotechnology and nanomedicine. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 9, e1447.
Suvanto, J., Nohynek, L., Seppänen-Laakso, T., Rischer, H., Salminen, JP., Puupponen-Pimiä, R. (2017) Variability in the production of tannins and other polyphenols in cell cultures of 12 Nordic plant species. Planta 246, 227-241
Tiu, BDB., Kernan, DL., Tiu, SB., Wen, AM., Zheng, Y., Pokorski, JK., Advincula, RC., Steinmetz, NF. (2017) Electrostatic layer-by-layer construction of fibrous TMV biofilms. Nanoscale 9, 1580-1590
van Zyl, AR., Meyers, AE., Rybicki, EP. (2017) Development of plant-produced protein body vaccine candidates for bluetongue virus. BMC Biotechnology 17, 47
Veliz F.A., Ma Y., Molugu S.K., Tiu B.D.B., Stewart P.L., French R.H., Steinmetz N.F. (2017) Photon management through virus-programmed supramolecular arrays. Advanced Biosystems 1, 1700088
Wallat J.D., Wek K., Chariou P.L., Carpenter B.L., Ghiladi R.A., Steinmetz N.F., Pokorski J.K. (2017) Fluorinated polymer–photosensitizer conjugates enable improved generation of ROS for anticancer photodynamic therapy. Polymer Chemistry 8, 3195-3202
Wallat, JD., Czapar, AE., Wang, C., Wen, AM., Wek, KS., Yu, X., Steinmetz, NF., Pokorski, JK. (2017) Optical and magnetic resonance imaging using fluorous colloidal nanoparticles. Biomacromolecules 18, 103-112
Webster, GR., Teh, AY., Ma, JK. (2017) Synthetic gene design-The rationale for codon optimization and implications for molecular pharming in plants. Biotechnology and Bioengineering 114, 492-502
Yanez, RJR., Lamprecht, R., Granadillo, M., Weber, B., Torrens, I., Rybicki, EP., Hitzeroth, II. (2017) Expression optimization of a cell membrane-penetrating human papillomavirus type 16 therapeutic vaccine candidate in Nicotiana benthamiana. PLoS One 12, e0183177
Zhao, C., Gunawardana, M., Villinger, F., Baum, MM., Remedios-Chan, M., Moench, TR., Zeitlin, L., Whaley, KJ., Bohorov, O., Smith, TJ., Anderson, DJ. (2017) Pharmacokinetics and preliminary safety of pod-intravaginal rings delivering the monoclonal antibody VRC01-N for HIV prophylaxis in a macaque model. Antimicrobial Agents and Chemotherapy 61, e02465-16.
Zhu, H., Reynolds, LB., Menassa, R. (2017) A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates. BMC Biotechnology 17, 53

2016

Arfi, ZA., Hellwig, S., Drossard, J., Fischer, R., Buyel, JF. (2016) Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins. Biotechnology Journal 11, 507-518
Boes A., Spiegel H., Kastilan R., Bethke S., Voepel N., Chudobová I., Bolscher JM., Dechering KJ., Fendel R., Buyel JF., Reimann A., Schillberg S., Fischer R. (2016) Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail. Malaria Journal 15, 279
Buyel, JF. (2016) Procedure to evaluate the efficiency of flocculants for the removal of dispersed particles from plant extracts. Journal of Visualized Experiments 2016 (110), 53940
Buyel, JF., Gruchow, HM., Tödter, N., Wehner, M. (2016) Determination of the thermal properties of leaves by non-invasive contact‑free laser probing. Journal of Biotechnology 217, 100-108
Buyel, JF., Hubbuch, J., Fischer, R. (2016) Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts. Journal of Visualized Experiments 2016 (114), 54343
Cuenca S, Mansilla C, Aguado M, Yuste-Calvo C, Sánchez F, Sánchez-Montero JM, Ponz F. (2016) Nanonets Derived from Turnip Mosaic Virus as Scaffolds for Increased Enzymatic Activity of Immobilized Candida antarctica Lipase B. Frontiers in Plant Science 7, 464
Hadad, R., Marks, E., Kalbina, I., Schön, K., Unemo, M., Lycke, N., Strid, Å., Andersson, S. (2016) Protection against genital tract Chlamydia trachomatis infection following intranasal immunization with a novel recombinant MOMP VS2/4 antigen. APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica 124, 1078-1086
Häkkinen, ST., E. Moyano, E., Cusidó, R-M., Oksman-Caldentey, K-M. (2016) Exploring the metabolic stability of engineered hairy roots after 16 years maintenance. Frontiers in Plant Science 7, 1486
Hansson, C., Schön, K., Kalbina, I., Strid, Å., Andersson, S., Bokarewa, MI., Lycke, NY. (2016) Feeding transgenic plants that express a tolerogenic fusion protein effectively protects against arthritis. Plant biotechnology Journal 14, 1106-1115
Kalbina, I., Lagerqvist, N., Moiane, B., Ahlm, C., Andersson, S., Strid, Å., Falk, KI. (2016) Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants. Protein Expression and Purification 127, 61-67
Menzel, S., Holland, T., Boes, A., Spiegel, H., Bolzenius, J., Fischer, R., Buyel, JF. (2016) Optimized blanching reduces the host cell protein content and substantially enhances the recovery and stability of two plant-derived malaria vaccine candidates. Frontiers in Plant Science 7, 159
Pirttimaa, M., Nasereddin, A., Kopelyanskiy, Kaiser, M., Yli-Kauhaluoma, J., Oksman-Caldentey, K-M., Brun, R., Jaffe, CL., Moreira, VM. (2016) Abietane-type diterpenoid amides with highly potent and selective activity against Leishmania donovani and Trypanosoma cruzi. Journal of Natural Products 79, 362-368
Puupponen-Pimiä, R., Nohynek, L., Juvonen, R., Kössö, T., Truchado, P., Westerlund-Wikström, B., Leppänen, T., Moilanen, E., Oksman-Caldentey, K-M. (2016) Fermentation and dry fractionation increase bioactivity of cloudberry (Rubus chamaemorus). Food Chemistry 197, 950-958
Zischewski, J., Sack, M., Fischer, R. (2016) Overcoming low yields of plant-made antibodies by a protein engineering approach. Biotechnology Journal 11, 107-116

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